Immunochemical quantitation of enzymes in human diploid cell line WI-38 |
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Authors: | Vita Milisauskas N. R. Rose |
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Affiliation: | The Center for Immunology, 203 Sherman Hall, School of Medicine, State University of New York at Buffalo, Buffalo, N.Y. 14214, USA |
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Abstract: | A quantitative immunochemical study was carried out of four enzymes, cathodal esterase, acid phosphatase, glucosaminidase and β-glucuronidase. In homogenates of the human diploid cell line WI-38, the relative amounts of the enzymes increased with the passage number of the culture, although great variation was found in later passages just before death of the culture. |
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