苦瓜提取物抗病毒作用及其有效成份的研究

本实验发现,小鼠皮下感染乙型脑炎病毒前腹腔注射苦瓜提取物(简称E.M.c.)有显著的保护作用,其保护率平均为66％;以E.M.c.静脉注射家兔后2小时,血清中的干扰素达高峰,其干扰素为I型干扰素;E.M.c.对小鼠NK细胞有明显的激活作用;经鉴定,E.M.c.中的有效成份主要是dsRNA,此外,还含有多糖类物质。

Studies on Antiviral Activity of the Extract of Monordica Charantca and its Active Principle

The extract of Monordica charantia (E. M. c.) was found to have a significant protective effect on Japanese B encephlitis virus infection in vivo. When mice were administered intraperitoneally with a dose of 0.4 mg E. M. c. at 18 hr, 12 hr, 4 hr before subcutaneous challenge with the virus respectively, the protective rate of treated group is 66%. E.M.c. was demonstrated as an interferon inducer. Type I IFN was found in rabbit serum and its level peaked at 2hr after intravenus injection of E. M.c. E.M.c. enhnnced the NK cell activity in mice significantly. Using cellulose fiber(CF-11) chromatography a double stranded RNA (termed as E.M.c.-dsRNA) was purified from E.M.c. E.M.c.-dsRNA was active at nanogram level in inducing resistance to viral infection in vitro. These results indicate that the main active principle in E.M.c, is a kind of dsRNA, Besides, the preliminary data suggest that a polysaccharide is present in E.M.c. in addition to the dsRNA, its antiviral activity is under study.