| C6／36细胞上登革2型病毒受体的免疫共沉淀 |
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| 引用本文： | 刘美德,赵彤言,董言德,陆宝麟.C6／36细胞上登革2型病毒受体的免疫共沉淀[J].Virologica Sinica,2004,19(3):217-219. |
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| 作者姓名： | 刘美德 赵彤言 董言德 陆宝麟 |
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| 作者单位： | 军事医学科学院微生物流行病研究所全军媒介生物重点实验室 北京100071
(刘美德，赵彤言，董言德)，军事医学科学院微生物流行病研究所全军媒介生物重点实验室 北京100071(陆宝麟) |
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| 基金项目： | 中国人民解放军总后勤部“九五”医学杰出中青年基金课题 |
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| 摘 要： | 本文利用差速离心方法提纯的登革2型病毒、抗登革病毒单抗、病毒受体、耦合有G蛋白的琼脂糖珠之间特异性结合的作用，利用免疫共沉淀方法从C6／36细胞中分离鉴定出分子量约为35kDa受体蛋白；并用糖蛋白染色方法否定了此蛋白的糖基化特征。
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| 关 键 词： | 登革2型病毒 C6／36细胞 受体 免疫共沉淀 糖蛋白 |
Immunological Co-sedimentation of Dengue 2 Virus' Receptor on the C6/36 Cells |
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| LIU Mei-de,ZHAO Tong-yan,DONG Yan-de,LU Bao-lin.Immunological Co-sedimentation of Dengue 2 Virus' Receptor on the C6/36 Cells[J].中国病毒学(英文版),2004,19(3):217-219. |
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| Authors: | LIU Mei-de ZHAO Tong-yan DONG Yan-de LU Bao-lin |
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| Institution: | LIU Mei-de,ZHAO Tong-yan**,DONG Yan-de,LU Bao-lin |
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| Abstract: | In this paper, based on the especial interaction among purificated Dengue 2 virus, monoclon- al antibody against Dengue virus, virus receptor, and Protein-G-Agrose; one non-glyprotein, which molecular weigt is approximately 35kDa, has been identified to be the receptor of Dengue 2 virus on the C6/36 cells by the method of immunological co-sedimentation. The glyprotein nature of this receptor is also denied through glyprotein stain procedure. |
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| Keywords: | Dengue 2 virus C6/36 cells Receptor Immunological co-sedimentation lyprotein |
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