Optimal Conditions for the Retrieval of CD4 and CD8 Antigens in Formalin-Fixed, Paraffin-Embedded Tissues |
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| Authors: | Seok H Kim Myeong C Kook Hyung G Song |
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| Institution: | Department of Pathology, Chungbuk National University College of Medicine, 62 Kaesin-dong, Cheongju, Chungbuk, South Korea. |
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| Abstract: | The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective. |
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