Localization of mannan at the surface of yeast protoplasts by scanning electron microscopy |
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Authors: | Marc Horisberger Jacqueline Rosset Heinz Bauer |
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Institution: | (1) Research Department, Nestlé Products Technical Assistance Co. Ltd., CH-1814 La Tour-de-Peilz, Switzerland;(2) Present address: NORDRECO AB, S-267 00 Bjuv, Sweden |
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Abstract: | The (13)glucanase of Basidiomycete QM 806 was used to prepare Saccharomyces cerevisiae and Candida utilis protoplasts. Plasma membranes isolated from S. cerevisiae contained a small amount of mannose and traces of glucose and ribose. Randomly distributed -mannan was detected by scanning electron microscopy at the surface of prefixed protoplasts using colloidal gold labelled with Concanavalin A as a marker. C. utilis protoplasts were also marked with anti-mannan antibodies. Again the distribution of mannan was random. This experiment indicated also that plasma membrane mannan has the same immunochemical determinants as cell wall mannan. It is hypothesized that mannan is mainly located in the outer layer of plasma membranes. |
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Keywords: | Candida utilis Saccharomyces cerevisiae Colloidal gold Cytochemistry Mannan Plasma membranes Scanning electron microscopy |
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