Temperature-adaptive differences in the kinetic properties of muscle-type lactate dehydrogenases of congeneric rocky-shore fishes from the Sea of Cortez |
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| Institution: | 1. College of Marine Science, University of South Florida, 140 7th Avenue South, St. Petersburg, FL 33701, USA;2. Florida Fish and Wildlife Conservation Commission, Fish and Wildlife Research Institute, 100 8th Avenue SE, St. Petersburg, FL 33701, USA;3. Polar Oceans Research Group, PO Box 368, Sheridan, MT 59749, USA;4. Center for Quantitative Fisheries Ecology, Old Dominion University, 800 West 46th St., Norfolk, VA 23508, USA;5. Department of Comparative Biomedicine and Food Science, Agripolis, University of Padova, Viale dell’Università, 16, I-35020 Legnaro (Pd), Italy;6. Department of Biology, University of Padova, Via G. Colomobo 3, 35121, Padova, Italy;1. Israel Oceanographic and Limnological Research Institute, The National Center for Mariculture, P.O. Box 1212, Eilat 88112, Israel;2. Ben Gurion University, The Faculty of Natural Sciences, P.O. Box 653, Beer-Sheva 84105, Israel;3. Ministry of Agriculture and Rural Development, P.O. Box 30, Bet Dagan 50250, Israel |
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| Abstract: | Four species of groupers, genus Epinephelus, exhibiting overlapping distribution ranges in the middle and lower regions of the Sea of Cortez, were studied in terms of biochemical, genetic, and functional enzymic features. The results of enzymatic assays of muscle-type lactate dehydrogenases (M4-LDH) from tropical-, subtropical- and temperate-zone groupers showed thermal compensatory differences in their kinetic properties (apparent Km of pyruvate and catalytic rate constant, kcat). These kinetic adaptations are reflected in a strong conservation of the functional characteristics of the enzyme at the mean temperature of their habitats which differ by 3–6°C on the average. These results point out that minor differences in habitat (body) temperature are sufficient to favor the evolution of functional adaptations in the enzymes of the species. The use of closely related congeneric species inhabiting different thermal environments is a valuable approach to the study of molecular evolution at a fine scale level. |
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