Physiological aspects of nm-g mutant: An ecdysteroid-deficient mutant of the silkworm,Bombyx mori |
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| Institution: | 1. Laboratory of Sericultural Sciences, Department of Agrobiology, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;1. Sericultural Experiment Station, Kobuchizawa, Yamanashi 409-16, Japan;1. Laboratory of Analytical & Molecular Toxicology (Forensic Chemistry and Toxicology laboratory), Institute of Forensic Science, Gujarat Forensic Sciences University, Gandhinagar, Gujarat, India;2. Pesticide Toxicology Division, National Institute of Occupational Health, Ahmedabad, Gujarat, India;1. School of Chemistry and Chemical Engineering, Hefei University of Technology, 230009, Hefei, Anhui, PR China;2. School of Food and Biological Engineering, Hefei University of Technology, 230009, Hefei, Anhui, PR China;3. Anhui Institute of Agro-Products Intensive Processing Technology, 230009, Hefei, Anhui, PR China;1. Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China;2. University of Chinese Academy of Sciences, Beijing, 100049, China;3. Shenyang Agricultural Environment Monitoring Station, Shenyang, 110016, China;1. School of Biotechnology, Southwest University, Chongqing 400716, China;2. School of Life Sciences, Southwest University, Chongqing 400716, China |
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| Abstract: | We examined physiological and endocrinological properties of the nm-g mutant of the silkworm, whose development was arrested at 1st or 2nd larval instar. The larva of the nm-g homozygote continued to feed and grow past the stage when a normal larva moulted to the next instar. The nm-g larva attained more than twice the body weight of a normal moulting larva, but remained in the same instar with no sign of the moulting until it finally died. The ecdysteroid concentration in the haemolymph of the nm-g larva was reduced and an increase in the titre, which occurred before moulting in the normal larva, was not observed. Injection of 20-hydroxyecdysone induced the nm-g larva to moult and ecdyse to the next larval instar. From these results, we conclude that nm-g is an ecdysteroid-deficient mutant. |
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