Binding of the bovine pancreatic secretory trypsin inhibitor (Kazal) to bovine serine (pro)enzymes |
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| Affiliation: | 1. Centro de Pesquisas em Biologia Molecular e Funcional (CPBMF) and Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga 6681 – Prédio 92A Tecnopuc, 90619-900 Porto Alegre, RS, Brazil;2. Programa de Pós-Graduação em Medicina e Ciências da Saúde, PUCRS, Porto Alegre, Brazil;3. Instituto de Toxicologia e Farmacologia, PUCRS, Porto Alegre, Brazil;4. Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;5. Programa de Pós-Graduação em Biologia Celular e Molecular, PUCRS, Porto Alegre, Brazil;6. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona–ISGlobal, Universitat de Barcelona, Barcelona, Spain;7. Fundação Oswaldo Cruz, Instituto de Tecnologia de Fármacos (Farmanguinhos), Rio de Janeiro, Brazil;8. Programa de Pós-Graduação em Química, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;9. Faculdade de Odontologia, PUCRS, Porto Alegre, Brazil;1. Department of Sciences, Roma Tre University, Viale Guglielmo Marconi 446, 00146 Roma, Italy;2. Technical Scientific Service and Core Facilities, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy;3. Department of Clinical Sciences and Translational Medicine, University of Roma “Tor Vergata”, Via Montpellier 1, I-00133 Roma, Italy;4. Centro de Investigatiòns Cientìficas Avanzadas (CIA), Departamento de Quìmica, Facultade de Ciencias, Universidad da Coruña, 15071 A Coruña, Galicia, Spain;5. Department of Physics, University of Genova, Via Dodecaneso 33, 16100 Genova, Italy;6. Interdepartmental Laboratory of Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, I-00146 Roma, Italy;1. School of Health Sciences, Purdue University, West Lafayette, IN, USA;2. Public Health Graduate Program, Purdue University, West Lafayette, IN, USA;3. Department of Cancer Epidemiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA;4. Department of Radiation Oncology, School of Medicine, Emory University, Atlanta, GA, USA;5. Department of Radiation Oncology, University of Arizona, Tucson, AZ, USA;6. Department of Neurology, Indiana University School of Medicine, Indianapolis, IN, USA;7. School of Public Health, Zunyi Medical University, Zunyi, Guizhou, China;8. Zunyi Medical and Pharmaceutical College, Zunyi, Guizhou, China |
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| Abstract: | The effect of temperature and pH on the association equilibrium constant (Ka) for the binding of the bovine pancreatic secretory trypsin inhibitor (bovine PSTI, type I; Kazal inhibitor) to bovine β-trypsin, bovine α-chymotrypsin and bovine trypsinogen has been investigated. The results suggest that serine (pro)enzyme inhibitor interaction involves both rigorous spatial configuration and molecular flexibility. |
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