Some extrachromosomal circular DNAs from Drosophila embryos are homologous to tandemly repeated genes |
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| Affiliation: | 1. Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Center for Quantitative Biology (CQB), Peking University, Beijing, China;2. Peking University Chengdu Academy for Advanced Interdisciplinary Biotechnologies, Chengdu, China;2. Animal Breeding and Genomics Centre, Wageningen UR Livestock Research, 6700 AH Wageningen, the Netherlands;3. VikingGenetics, Assentoft, DK-8960 Randers, Denmark;1. Paul F. Glenn Center for Biology of Aging Research, Department of Genetics, Blavatnik Institute, Harvard Medical School (HMS), Boston, MA, USA;2. Department of Ophthalmology, Department of Neuropsychiatry, Keio University School of Medicine, Tokyo, Japan;3. IIIUC-Institute of Interdisciplinary Research, University of Coimbra, Coimbra, Portugal;4. Computational Biology Department, Carnegie Mellon University, Pittsburgh, PA, USA;5. Cantata/Dovetail Genomics, Scotts Valley, CA, USA;6. Zymo Research Corporation, Irvine, CA, USA;7. Department of Medicine, Brigham and Women’s Hospital, HMS, Boston, MA, USA;8. Division of Nephrology, University of Washington, Seattle, WA, USA;9. Georgetown University School of Medicine, Washington, DC, USA;10. Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA, USA;11. Experimental Gerontology Section, NIA/NIH, Baltimore, MD, USA;12. Department of Pharmacology, UNSW, Sydney, NSW, Australia;13. Department of Biomolecular Engineering, UCSC, Santa Cruz, CA, USA;14. Department of Stem Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan;15. Vium, San Mateo, CA, USA;16. Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, HMS, Boston, MA, USA;17. The Massachusetts General Hospital Cancer Center, HMS, Boston, MA, USA;18. Department of Genetics, HMS, Boston, MA, USA;19. Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, BioQuant, Heidelberg, Germany;20. Paul F. Glenn Center for Biology of Aging Research, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA;21. Division of Geriatrics, University of Nebraska Medical Center, Durham Research Center II, Omaha, NE, USA;22. Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal;23. Department of Pathology, University of Washington, Seattle, WA, USA;24. Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA;25. Department of Pathology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA, USA;26. Laboratory of Receptor Biology and Gene Expression, NCI, NIH, Bethesda, MD, USA;27. Joslin Diabetes Center, Boston, MA, USA;1. Laboratory of Plant Physiology “Coaracy M. Franco”, Center R&D in Ecophysiology and Biophysics, Agronomic Institute (IAC), Campinas, SP, Brazil;2. Centre for Research in Biosciences, University of the West of England (UWE), Bristol, UK;3. Department of Plant Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil;4. School of Biological Sciences, Institute of Molecular Plant Sciences, University of Edinburgh, Edinburgh, UK;1. Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, the Netherlands;2. Department of Vascular Surgery, Erasmus University Medical Center, Rotterdam, the Netherlands;3. Department of Radiotherapy, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, the Netherlands |
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| Abstract: | In Drosophila melanogaster embryos we have identified three classes of extrachromosomal circular DNA molecules homologous to the three main families of tandemly repeated genes, 5 S, rDNA and histone. 5 S genes are present in circular multimeric molecules containing up to 16 copies of the 375(±7) base-pair repeated unit. Circular molecules homologous to rDNA are also multimeric molecules, which contain up to ten copies of the 240 base-pair tandemly repeated sequence of the non-transcribed spacer. The two major genomic classes of histone units (4800 and 5000 bases) are found only as monomeric circular molecules.No circular intermediate of the I transposable element was detected in embryos laid by f1 dysgenic females produced by the I-R system of hybrid dysgenesis.As far as we know, it is the first time that genes have been identified among extrachromosomal circular molecules independently of any specific amplification phenomenon. |
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