Detection and Identification by PCR of a Highly Virulent Phylogenetic Subgroup among Extraintestinal Pathogenic Escherichia coli B2 Strains |
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Authors: | Philippe Bidet, Arnaud Metais, Farah Mahjoub-Messai, Lionel Durand, Marie Dehem, Yannick Aujard, Edouard Bingen, Xavier Nassif, St phane Bonacorsi |
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Affiliation: | Philippe Bidet, Arnaud Metais, Farah Mahjoub-Messai, Lionel Durand, Marie Dehem, Yannick Aujard, Edouard Bingen, Xavier Nassif, and Stéphane Bonacorsi |
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Abstract: | Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches. |
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