Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India |
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| Authors: | Paramageetham Ch Prasad Babu G Rao JVS |
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| Institution: | (1) Plant Biotechnology Laboratory, Department of Botany, S.V. University, Tirupati-, 517 502, India |
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| Abstract: | Leaf segments excised from Centella asiatica, a medicinal and neutraceutical plant, produced abundant somaticembryoswhen cultured onMS mediumwith 9.29  Mkinetin in combination with 2.26 M2,4-D. Granular, white,shiny clusters of callus developed after 1 week of culture, and then formed heart and cotyledonary stage embryoson the same medium after 4 weeks. Somatic embryos matured and germinated in the presence of MS mediumcontaining 2.32 M kinetin with (2.89M) GA3. Plantlets were successfully transferred to pots containing amixture of soil and vermiculite (1:1). |
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| Keywords: | Apiaceae asiaticosides bipolar and unipolar embryos leaf explants |
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