Thermal analysis of bacteriophage T4. |
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Authors: | Y Kawai H Takahashi T Fujita S Koga |
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Affiliation: | The Institute of Applied Microbiology, University of Tokyo, Tokyo, Japan |
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Abstract: | The process of bacteriophage T4 morphogenesis was studied using a heat leakage scanning calorimeter. Thermograms of defective mutant 49 (am NG727) in permissive and non-permissive cells of showed a difference in thermal properties between packaged and non-packaged DNA molecules. , non-packaged DNA carried out their thermal transition at 85°C, the same temperature as that of T4 DNA melting measured in the standard saline citrate buffer, while the packaged DNA gave a sharper peak at 87°C due to some interaction with the head shell structure. Empty head shells showed a sharp heat absorption peak at 89°C both and , indicating the high degree of cooperativity in their conformational changes. |
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