Molecular cloning and functional characterization of two alternatively spliced Ntcp isoforms from mouse liver1 |
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Authors: | Cattori V Eckhardt U Hagenbuch B |
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Institution: | Division of Clinical Pharmacology and Toxicology, Department of Medicine, University Hospital, CH-8091, Zurich, Switzerland. |
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Abstract: | To isolate the murine Na+/taurocholate cotransporting polypeptide (Ntcp), we screened a mouse liver cDNA library and identified Ntcp1, encoding a 362 amino acid protein and Ntcp2, encoding a 317 amino acid protein which had a shorter C-terminal end. Both isoforms mediated saturable Na+-dependent transport of taurocholate when expressed in Xenopus laevis oocytes. Analysis of the gene revealed that Ntcp2 is produced by alternative splicing where the last intron is retained. |
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