| 层析聚焦法分离纯化末端脱氧核苷酰转移酶及其抗血清制备和鉴定 |
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| 引用本文： | 李敏,孙芝琳.层析聚焦法分离纯化末端脱氧核苷酰转移酶及其抗血清制备和鉴定[J].中国生物化学与分子生物学报,1988,4(2):124-129. |
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| 作者姓名： | 李敏 孙芝琳 |
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| 作者单位： | 华西医科大学生化教研室 成都
(李敏)，华西医科大学生化教研室 成都(孙芝琳) |
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| 摘 要： | 应用层析聚焦和Oligo(dT)-纤维素亲和层析相结合的方法,从小牛胸腺中分离纯化末端脱氧核苷酰转移酶(TdT)。纯化的TdT聚丙烯酰胺凝胶电泳呈一条区带,SDS-聚丙烯酰胺凝胶电泳分子量为24,000及26,000d的两条区带。此纯化TdT径戊二醛交联法,自身交联后免疫家兔,得到兔抗小牛TdT的单价抗血清,并进行了免疫学鉴定。
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| 关 键 词： | 小牛胸腺 末端脱氧核苷酰转移酶 层析聚焦 抗TdT血清 |
| 收稿时间： | 1988-04-20 |
ISOLATION AND PURIFICATION OF TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE BY USING CHROMATOFOCUSING AND PREPARATION AND IDENTIFICATION OF ANTISERUM |
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| Li,Min Sun,Zhi-lin.ISOLATION AND PURIFICATION OF TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE BY USING CHROMATOFOCUSING AND PREPARATION AND IDENTIFICATION OF ANTISERUM[J].Chinese Journal of Biochemistry and Molecular Biology,1988,4(2):124-129. |
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| Authors: | Li Min Sun Zhi-lin |
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| Institution: | (Department of biochemistry, west china university of medical sciences, Chengdu |
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| Abstract: | Using a combined method of chromatofocusing and Oligo(dT)-celluslose affinity chromatography, the terminal deoxynucleotidyl transferase (TdT) isolated from calf thymus was purified. The purified enzyme exhibited a single zone in 10% polyacrylamide gel electrophoresis, and contained two polypeptides with molecular weight of about 24,000 and 26,000 dalton respectively.Antibody against the purified TdT from calf thymus has been prepared from glutaric dialdehyde crosslinked TdT. The antiserum was identified with crude extracts of calf and ox tissue. |
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| Keywords: | Calf thymus Terminal deoxynucleotidyl transferase Chromatofocu-sing Antiserum |
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