Concepts related to salt resistant estradiol receptors in rat uterine nuclei: nuclear matrix |
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Authors: | E R Barrack E F Hawkins S L Allen L L Hicks D S Coffey |
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Affiliation: | Department of Pharmacology and Experimental Therapeutics, The James Buchanan Brady Urological Institute, Department of Urology and The Oncology Center The Johns Hopkins University School of Medicine Baltimore, Maryland 21205 USA |
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Abstract: | When 3H-estradiol (0.1 μg) is injected into immature female rats, virtually all of the label that is recovered with uterine nuclei can be solubilized by 0.6 M KCl. Salt resistant uterine nuclear estrogen binding sites do not become labeled within one hour after the injection of 3H-estradiol, but these sites do exist and can be revealed when isolated nuclei are subjected to an estradiol exchange assay. These saturable, high affinity salt resistant sites appear to be associated with the uterine nuclear matrix, a residual structure of the nucleus. |
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