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Preparation of Fine Magnetic Particles and Application for Enzyme Immobilization
Authors:Masashige Shinkai   Hiroyuki Honda  Takeshi Kobayashi
Affiliation: a Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan
Abstract:Fine magnetic particles (ferrofluid) were prepared from a co-precipitation method by oxidation of Fe2+ with nitrite. The particles were activated with (3-aminopropyl)triethoxysilane in toluene and the activated particles were combined with some enzymes by using glutaraldehyde. Enzyme-immobilized magnetic particles were between 4-70 nm and the size could be changed corresponding to the ratio of the amount of Fe2+ to that of nitrite. In the immobilization of β-glucosidase, activity yield was 83% and 168 mg protein was immobilized per g magnetite. Other enzymes or proteins could be immobilized at the level between about 70 and 200mg/g support. Immobilized β-glucosidase was stable at 4°C. Magnetic particles immobilized with β-glucosidase responded quickly to the magnetic field and “ON-OFF” control of the enzyme reaction was possible.
Keywords:Ferrofluid  magnetic particles  aminosilane  glutaraldehyde  enzyme immobilization  covalent binding
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