盾叶薯蓣花药培养及单倍体植株的获得

以处于单核期的盾叶薯蓣花药为材料,经愈伤组织途径成功获得单倍体盾叶薯蓣新材料。对盾叶薯蓣花药培养、植株再生过程的研究表明:来自不同居群的外植体对花药愈伤组织诱导有显著影响;W14是适合花药愈伤组织诱导的基本培养基;花药愈伤组织增殖和分化的适宜培养基为:MS基本培养基 BA2.0mg/L IAA0.2mg/L;生根培养基为添加了IBA2.0mg/L、NAA0.4mg/L和0.5g/L活性碳的MS培养基。以流式细胞仪和根尖染色体压片法检查花培植株的结果表明,有8%～12%的个体为单倍体。实验结果为进一步开展单倍体育种及相关理论研究提供了材料和技术基础。

Haploid Callus and Plantlets Regenerated from Anther Culture of Dioscorea zingiberensis (Dioscoreaceae)

Haploid plantlet and callus were induced from the anther culture of Dioscorea zingiberensis as the explant at the stag of later uninucleate period.The results indicates that the basal culture media and explants from different population affect dramatically callus induction from anthers.W14 was the best basal media for the first step of callus induction.MS media with BA2.0 mg/L IAA0.2 mg/L improved callus' multiplication and differentiation.MS media with 2.0 mg/L IBA,0.4 mg/L NAA and 0.5 g/L active carbon were suitable for rooting.Examination of the anther cultured plantlet displayed that 8%-12% clones were haploid.The established technique system and obtained haploid plant would provide solid support and valuable materials for further breeding of Dioscorea zingiberensis.