High-Resolution Detection of Five Frequencies in a Single 3D Spectrum: HNHCACO - a Bidirectional Coherence Transfer Experiment |
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| Authors: | Yuxi Pang Lei Zeng Alexander V. Kurochkin Erik R.P. Zuiderweg |
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| Affiliation: | (1) Biophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A;(2) Department of Biological Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A.;(3) Department of Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A |
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| Abstract: | A new triple-resonance pulse sequence, 3D HNHCACO, is introduced and discussed, which identifies sequential correlations of the backbone nuclei (H (i-1), C(i-1), C (i-1), NH(i)) of doubly labeled proteins in H2O. The three-dimensional (3D) method utilizes a recording of 15N and 13C resonances in a single indirect time domain, the 13C resonance in another indirect time domain, and detects both NH and H protons. A bidirectional coherence transfer (NH(i)  N(i) C(i-1) C(i-1) H(i-1)) is effectuated, resulting in a single high-resolution 3D spectrum that contains the frequencies of all five backbone nuclei. The experiment was applied to the 12.3 kDa ribonuclease from Bacillus intermedius (Binase). |
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| Keywords: | bidirectional coherence transfer Binase gradient coherence selection HNHCACO simultaneous 15N and 13C![]("</a) /content/u5806t755607283u/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" > acquisition |
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