Soluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth |
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| Authors: | Yukihiko Arikawa Keiichiro Enomoto Haruhiro Muratsubaki Mitsuo Okazaki |
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| Institution: | Food Technology Research Institute of Nagano Prefecture, 205-1 Nishibanba, Kurita, Nagano City 380-0921, Japan;Department of Clinical Biochemistry, Kyorin University School of Health Sciences, Miyashita, Hachioji, Tokyo 192-0005, Japan;Gene Research Center Shinshu University, 3-15-1 Tokida, Ueda City, Nagano 386-0018, Japan |
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| Abstract: | In Saccharomyces cerevisiae, the cytosolic and promitochondrial isoenzymes of fumarate reductase are encoded by the FRDS and OSM1 genes, respectively. The product of the OSM1 gene is reported to be required for growth in hypertonic medium. Simultaneous disruption of the FRDS and OSM1 genes resulted in the inability of the yeasts to grow anaerobically on glucose as a carbon source, and disruption of the OSM1 gene caused poor growth under anaerobic conditions. However, the disruption of both the FRDS and/or OSM1 genes had no effect on aerobic growth or growth under hypertonic conditions. These results suggest that the fumarate reductase isoenzymes in Saccharomyces cerevisiae are essential for anaerobic growth but not for growth under hypertonic conditions. |
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| Keywords: | Fumarate reductase OSM1 gene Promitochondrial enzyme Yeast |
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