Discrete localization of various fatty-acid-binding proteins in various cell populations of mouse retina |
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Authors: | Sachiko Saino-Saito Reza Mohammad Nourani Hiroo Iwasa Hisatake Kondo Yuji Owada |
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Institution: | (1) Section of Histology and Medical Embryology, Department of Human Morphology and Applied Biology, University of Pisa, Via Roma 55, 56126 Pisa, Italy;(2) Department of Internal Medicine and Gastroenterology, University of Bologna, Bologna, Italy;(3) Division of Pharmacology and Chemotherapy, Department of Internal Medicine, University of Pisa, Pisa, Italy;(4) Section of Pathology, Department of Surgery, University of Pisa, Pisa, Italy |
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Abstract: | The analysis of myenteric neurons is becoming increasingly important for the assessment of enteric nervous system injury and
degeneration occurring in motor disorders of the gut. Limited information is presently available on the quantitative estimation
of myenteric neurons and glial cells in paraffin-embedded colonic sections; additional data would be useful for diagnostic
purposes. In this morphometric study, we performed immunohistochemistry to count myenteric neurons and glial cells in paraffin
sections of human colon. Serial cross sections of formalin-fixed paraffin-embedded full-thickness normal human left colon
(n = 10, age-range: 50–72 years) were examined. HuC/D and S100β antigens were found to be the best markers for the detection
of neurons and glial cells, respectively. Significant correlations were noted between the numbers of neurons/glial cells and
the respective myenteric ganglion areas. These findings suggest that HuC/D-S100β-immunostained paraffin cross sections of
human colon can be regarded as valuable tools for the quantitative estimation of myenteric neurons and glial cells. Based
on the present method, only a limited number of paraffin sections are needed for reliable quantitative assessments of myenteric
ganglion cells, thus allowing fast and simple approaches in the settings of the histopathological diagnosis of colonic motility
disorders and retrospective evaluations of pathological archival tissue specimens.
R. De Giorgio is the recipient of grants from the Fondazione Del Monte di Bologna e Ravenna and from the Fondazione Cassa
di Risparmio, Bologna, Italy. The authors declare no conflicting interests. |
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Keywords: | Enteric nervous system Colon Myenteric neurons Myenteric glial cells Paraffin-embedded tissue Human |
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