Impaired generation of taurine chloramine by synovial fluid neutrophils of rheumatoid arthritis patients |
| |
Authors: | Kontny E Wojtecka-ŁUkasik E Rell-Bakalarska K Dziewczopolski W Maśliński W Maślinski S |
| |
Institution: | (1) Department of Pathophysiology and Immunology, Institute of Rheumatology, Warsaw, Poland, PL;(2) Department of Biochemistry, Warsaw, Poland, PL;(3) Out-patient Department, Institute of Rheumatology, Warsaw, Poland, PL;(4) University School of Medicine, Warsaw, Poland, PL |
| |
Abstract: | Summary. Taurine (Tau), a dominant free amino acid present in neutrophil cytoplasm, serves as a scavenger for hypochlorous acid (HOCl)
released during these cells activation. The resulting taurine chloramine (Tau-Cl) exerts potent anti-inflammatory properties.
In the present study we tested the hypothesis that the formation of Tau-Cl is impaired in neutrophils isolated from rheumatoid
arthritis (RA) patients. The inhibition of zymosan-triggered chemiluminescence in the presence of exogenous Tau was used for
indirect measurement of Tau-Cl generation. The chemiluminescence of neutrophils isolated from peripheral blood (PB) of healthy
volunteers and RA patients was inhibited by Tau with similar potency. By contrast, synovial fluid (SF) neutrophils of these
patients were significantly less sensitive for Tau-mediated inhibition. Therefore, our data indicate impaired generation of
Tau-Cl in neutrophils isolated from SF of RA patients.
Received November 29, 2001 Accepted January 9, 2002 Published online August 30, 2002
Acknowledgements This work was supported by grants from the State Committee for Scientific Research of Poland (No. P05A 104 19) and the Institute
of Rheumatology. The Institute of Rheumatology is supported by a core grant from the State Committee for Scientific Research
of Poland.
Authors' address: Ewa Kontny, Ph.D, Department of Pathophysiology and Immunology, Institute of Rheumatology, Spartanska 1, 02-637 Warsaw, Poland,
E-mail: zpatiir@warman.com.pl
Abbreviations: Tau, taurine; Tau-Cl, taurine chloramine; PB, peripheral blood; SF, synovial fluid; RA, rheumatoid arthritis |
| |
Keywords: | : Rheumatoid arthritis Synovial fluid neutrophils Taurine chloramine |
本文献已被 PubMed SpringerLink 等数据库收录! |
|