Degradation of the framework of the Chlamydomonas cell wall by proteases present in a commercially available alpha-amylase preparation |
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Authors: | S H Imam W J Snell |
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Affiliation: | Department of Cell Biology, University of Texas Health Science Center, Dallas 75235. |
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Abstract: | A commercially available alpha-amylase derived from Bacillus licheniformis contained an enzymatic activity able to degrade the inner portion or framework of the cell wall of Chlamydomonas reinhardtii. Both the wall-degrading activity and the contaminating protease were destroyed by heating the alpha-amylase preparation at 90 degrees C for 30 min. Since the alpha-amylase activity was uneffected by heat treatment, we conclude that it was not the alpha-amylase but the contaminating protease in the preparation that was responsible for the cell wall-degrading activity. |
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