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Amplified somatic embryogenesis from male flowers of triploid banana and plantain cultivars (Musa spp.) |
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| Authors: | Jean-Vincent Escalant Claude Teisson François Cote |
| Affiliation: | 1. Unidad de Biotecnologia, Centro Agronomico de Investigacion y Ense?anza (CATIE), CIRAD-FLHOR, 7170, Turrialba, Costa Rica 2. Laboratoire BIOTROP, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), B.P. 5035, Montpellier, France |
| Abstract: | Summary Somatic embryogenesis and plant regeneration of banana and plantain cultivars (Musa spp.) were obtained by culturing young male flowers. Multiplication and maintenance of embryogenic cultures were achieved by culturing somatic embryos in a temporary immersion system (SIT). A multiplication rate of 40 allowed us to obtain more than 6000 somatic embryos after 6 mo. of subculture. Plant recovery frequencies were 60 to 70%. This method was expanded to different banana and plantain genomic groups. |
| Keywords: | banana plantain triploids somatic embryogenesis in vitro temporary immersion system plant regeneration |
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