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DISTRIBUTION OF TWO TYPES OF EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) IN THE NORTHEAST ATLANTIC REGION AS DETERMINED BY IMMUNOFLUORESCENCE AND COCCOLITH MORPHOLOGY1
Authors:Judith van Bleijswijk  Paul van der Wal  Rob Kempers  Marcel Veldhuis  Jeremy R Young  Gerard Muyzer  Elisabeth de Vrind-de Jong  Peter Westbroek
Abstract:Culture strains of Emiliania huxleyi (Lohmann 1902) Hay et al. 1967 were placed into two groups designated E. huxleyi type A and type B on the basis of coccolith morphology and immunological properties of the coccolith polysaccharide. We studied the distribution of these types in the North Atlantic region using an indirect immunofluorescence assay with antisera directed against the coccolith polysaccharide of E. huxleyi type A and type B and epifluorescence microscopy. In field samples taken in the Northeast Atlantic Ocean, E. huxleyi type A was found exclusively. In contrast, type B was dominant in the North Sea. Scanning electron microscopy of the samples revealed the same unequal distribution of the two types as found with the immunofluorescent-labelling assay.
Keywords:biogeography  coccoliths  Coccolithophoridae  Emiliania huxleyi  immunofluorescence  NE Atlantic Ocean  North Sea  phytoplankton  scanning electron microscopy
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