Iaa oxidase and polyphenol oxidase activities of peanut peroxidase isozymes |
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| Authors: | Om.P. Srivastava Robert B. Van Huystee |
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| Affiliation: | Department of Plant Sciences, University of Western Ontario, London, Ontario, Canada N6A 3K7 |
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| Abstract: | Four anionic isozymes (A1, A2, A4 and A5) from peanut cells in suspension medium possessed IAA oxidase and polyphenol oxidase activities. The specific activities of each of the enzymes differed among the 4 isozymes. The pH optima established in these assays for peroxidase was acidic, for IAA oxidase neutral and for polyphenol oxidase alkaline. All 4 isozymes had different Km and Vmax for the enzyme activities of peroxidase and polyphenol oxidase. The sigmoid kinetics from the IAA oxidase assays for the isozymes probably indicates an allosteric nature. |
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| Keywords: | peanut peroxidase IAA oxidase pH optima sigmoid kinetics. |
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