Extracellular hydroxyproline-rich glycoprotein of suspension-cultured tobacco cells |
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Authors: | Hidetaka Hori Sitiro Sato |
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Affiliation: | 1. Department of Botany, Faculty of Science, Hokkaido University, Sapporo 060, Japan;2. Department of Botany, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan |
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Abstract: | Hydroxyproline-rich glycoprotein was isolated from the used media of tobacco XD-6 cells cultured in suspension and purified by repeated ion exchange- and gel filtration-chromatography. The preparation was judged to be homogeneous from ultracentrifugation and isoelectric focusing. The dry wt of the glycoprotein was composed of 94% polysaccharide and 6% protein. Hydroxyproline accounted for 23% of the amino acids in the protein moiety. The polysaccharide moiety consisted of 44% galactose, 30% arabinose, 5% rhamnose, and 21% uronic acid. About 10% of the uronic acid residues were present as methyl esters. |
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Keywords: | tobacco cell extracellular glycoprotein suspension culture secretion hydroxyproline |
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