小鼠胚胎干细胞建系技术研究进展

目前,对小鼠胚胎干细胞的研究较为深入,并已成为研究细胞分化及信号转导、新基因发现及功能鉴定、器官发生、人类疾病和药物开发等的有效手段。胚胎干细胞建系是一项基础性工作。虽然技术日趋成熟,有些品系小鼠的胚胎干细胞建系已是常规技术,但不同品系小鼠胚胎干细胞的建系效率仍有很大差异,建系途径和方法各有特点,一个品系胚胎干细胞的建系方法不一定都适用于其他品系。本文从小鼠胚胎干细胞建系的途径、分离操作技术、培养体系等方面进行综述,并就与之相关的有些问题提出思考和对策。

Mouse Embryonic Stem Cells: Advances in Technology

Mouse embryonic stem cells,which are most intensively studied among all kinds of animal embryonic stem cells,have been widely used in studies of differentiation and signal transduction of cells,identification and function of new genes,organogenesis,pathogenesis of human diseases,pharmaceutical development.Establishment of embryonic stem cell lines is a kind of basic work,and its related technologies have been increasingly improved and have become the routine technologies in some strains.However,there exist significant differences in methods,schemes and efficiencies when different strains are used for developing embryonic stem cell lines.The methods for establishing embryonic stem cell lines of one strain of mice do not always work when other strains are used to establish embryonic stem cell lines,especially when used in different laboratories.This paper focuses on addressing technological advances in embryonic stem cell establishment,including methods,isolation procedures,culture systems,and pluripotency maintenance.