The Dual Effects of Ca2+ on Binding of the Glycolytic Enzymes,Phosphofructokinase and Aldolase,to Muscle Cytoskeleton |
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| Institution: | 1. Laboratory of Translational Medicine and Nanotherapeutics, Department of Human Genetics and Molecular Medicine, School of Health Sciences, Central University of Punjab, Bathinda, India;2. School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK;3. CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Telangana, India;4. Amity Institute of Biotechnology, Amity University Rajasthan, India;5. Department of Medical Lab Technology, University Institute of Applied Health Sciences, Chandigarh University, Mohali, India |
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| Abstract: | We show here that in rat diaphragm muscle, a short time of incubation with the Ca2+-ionophore A23187 induced an increase in cytoskeleton-bound phosphofructokinase (EC 2.7.1.11) and aldolase (EC 4.1.2.13), whereas a longer period of incubation, which causes a pathological rise in intracellular Ca2+, induced a decrease in bound enzymes. Lactate concentration correlated with both phases of Ca2+ action on the binding of the enzymes. The increase in cytoskeleton-bound enzymes could be prevented by treatment with the calmodulin antagonists trifluoperazine or CGS 9343B (a novel, potent, and selective inhibitor of calmodulin activity). These results suggest that calmodulin is involved in the Ca2+-induced binding of the enzymes to muscle cytoskeleton. |
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