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Intracellular Proteolytic Activity in Mycelia of Arthrobotrys oligospora Bearing Mycoparasitic or Nematode Trapping Structures
Institution:1. UMR ENTROPIE (Université de La Réunion, IRD, IFREMER, Université de Nouvelle-Calédonie, CNRS), Université de La Réunion, Saint-Denis, La Réunion, France;2. Laboratoire Cogitamus, Paris, France;3. Centre Sécurité Requin, Saint-Leu, La Réunion, France;4. Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO, Marseille, France;5. Squal’Idées, Saint-Leu, La Réunion, France;6. Laboratoire d′Excellence CORAIL, Perpignan, France;1. Department of Clinical Physiology, Translational Medicine, Malmö, Lund University, Malmö, Sweden;2. Department of Urology, University Clinic Carl Gustav Carus, Dresden, Germany;3. Department of Urology, Tampere University Hospital and University of Tampere, Tampere, Finland;4. Department of Urology, Azienda Ospedaliera S. Giuseppe Moscati, Avellino, Italy;5. Urology Department and Kidney Transplant Unit, Translational Research Group of Urology GITUR-IBSAL, Salamanca University Hospital, Salamanca, Spain;6. Department of Urology, Radboud University, Nijmegen Medical Centre, Nijmegen, The Netherlands;7. Department of Urology, Charité, Universitätsmedizin, Berlin, Germany;8. Department of Urology, Sant’Andrea Hospital, Sapienza University of Rome, Rome, Italy;9. Department of Urology, Andros Clinic, Arnhem, The Netherlands;10. London, UK;11. European Association of Urology, Research Foundation (EAU RF), Arnhem, The Netherlands;12. Region Västra Götaland, Sahlgrenska University Hospital, Department of Clinical Physiology, Gothenburg, Sweden;13. Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden;14. Department of Urology, Skåne University Hospital, Malmö, Lund University, Sweden;1. Assistant Professor, Department of Pediatrics, Yenepoya Medical College, Deralakatte, Mangalore, Karnataka, India;2. Post Graduate, Department of Anaesthesia, A.J. Institute of Medical Science, Mangalore, Karnataka, India;3. Associate Professor, Pediatrics, Yenepoya Medical College, Deralakatte, Mangalore, Karnataka, India;1. College of Life Science, Inner Mongolia University, Hohhot 010021, China;2. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China
Abstract:Persson, Y., and Friman, E. 1993. Intracellular proteolytic activity in mycelia of Arthrobotrys oligospora bearing mycoparasitic or nematode trapping structures. Experimental Mycology 17, 182-190. The fungus Arthrobotrys oligospora parasitizes other fungi with the aid of coils and captures and digests nematodes by means of adhesive traps. We have compared proteolytic activities of mycelial extracts from coils and traps with those of vegetative hyphae. A. oligospora produced a number of proteases active at both alkaline and acidic pH. Coil extract had significantly higher proteolytic activity than extracts of vegetative hyphae. Several coil culture-specific bands were found after substrate gel electrophoresis. Pepstatin-sensitive proteolytic activity at acidic pH was higher in coil extract than in normal mycelial extracts, although the total proteolytic activity was the same. No proteolytic activity was connected solely to mycelial extracts with traps and no enhancement of proteolytic activity was observed during infection of nematodes.
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