Electrophoretic Karyotypes of Fusarium spp. |
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| Institution: | 1. Virginia Commonwealth University, Robertson School of Media and Culture, Richmond, VA;2. University of Wisconsin - Milwaukee, Joseph J. Zilber School of Public Health, Milwaukee, WI;3. University of Minnesota, Hubbard School of Journalism and Mass Communication, Minneapolis, MN;4. Virginia Commonwealth University, School of Medicine, Richmond, VA;5. Virginia Commonwealth University, Department of Psychology, Richmond, VA;6. University of California - Irvine, Sue & Bill Gross School of Nursing, Irvine, CA;1. Centre de Recherche de l''Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Quebec City, Quebec, Canada;2. Département de Biochimie, de Microbiologie et de Bio-informatique, Faculté des Sciences et de Génie, Université Laval, Quebec City, Quebec, Canada;3. Université Clermont Auvergne, CNRS, SIGMA Clermont, ICCF, F-63000 Clermont-Ferrand, France;4. Université Clermont Auvergne, CNRS, Observatoire de Physique du Globe de Clermont-Ferrand, UAR 833, F-63000 Clermont-Ferrand, France;5. Université Clermont Auvergne, CNRS, Laboratoire de Météorologie Physique, UMR 6016, F-63000 Clermont-Ferrand, France |
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| Abstract: | Migheli, Q., Berio, T., and Gullino, M. L. 1993. Electrophoretic karyotypes of Fusarium spp. Experimental Mycology 17, 329-337. The electrophoretic karyotype of 17 antagonistic and pathogenic strains of Fusarium spp. has been established by using contour-clamped homogeneous electric field gel electrophoresis. Intact chromosomal DNA was prepared from fungal protoplasts with standard procedures. Up to 11 distinct chromosomal bands were resolved after 184 h of migration at 50 V. Polymorphic karyotypes were observed in different species of Fusarium, formae speciales of F. oxysporum , and races of F. oxysporum f.sp. dianthi. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards, the size of the Fusarium genome was estimated to range from approximately 18.1 to 51.5 Mb. The suitability of electrophoretic karyotyping as a tool for strain characterization, as well as some applications in hybridization analysis of Fusarium spp., is discussed. |
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