l-Serine production using a resting cell system of Hyphomicrobium strains |
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| Affiliation: | 1. Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Naka-machi, Nara 631, Japan;2. Department of Biotechnology, Faculty of Engineering, Tottori University, Tottori 680, Japan;1. Division of Nuclear Medicine, Department of Radiology, 2276 Med Sci I Bldg, SPC-5610, University of Michigan Medical School, Ann Arbor, MI-48109, USA;2. Department of Applied Chemistry, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Gyeonggi-do, 17140, Republic of Korea;3. Department of Nuclear Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 13620, Republic of Korea;4. Center for Nanomolecular Imaging and Innovative Drug Development, Advanced Institutes of Convergence Technology, Suwon, 16229, Republic of Korea;5. Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea;6. Department of Chemistry and Chemical Engineering, Inha University, 100 Inha-ro Nam-gu, Incheon, 402-751, Republic of Korea;1. Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey;2. Computational Biology and Molecular Simulations Laboratory, Department of Biophysics, School of Medicine, Bahcesehir University, 34349 Istanbul, Turkey;3. Dipartimento di Farmacia, Università “G. d’Annunzio” Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo, CH, Italy;1. Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute BS, 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, India;2. Microbiology Division, CSIR-Central Drug Research Institute BS 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, India;3. Academiy of Scientific and Innovative Research, New Delhi 110001, India;1. College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, Republic of Korea;2. School of Pharmacy, Wenzhou Medical College, 1210 University Town, Wenzhou, Zhejiang 325035, China;3. Wenzhou Undersun Biotechnology Co., Ltd, Wenzhou, Zhejiang, China;4. KIST Gangneung Institute, Gangneung 210-340, Republic of Korea;5. College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Jeonnam 534-729, Republic of Korea;1. College of Pharmacy, Dali University, Dali 671000, China;2. Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from Western Yunnan (Cultivation), Dali University, Dali 671000, China |
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| Abstract: | Twenty-six strains of methylotrophic hyphomicrobia were examined for their ability to produce l-serine from methanol and glycine in a resting cell reaction. l-Serine productivity of over 5 mg/ml was observed in 7 strains, and Hyphomicrobium sp. NCIB10099 was found to exhibit the highest productivity. Under optimized conditions using this bacterium, 45 mg/ml l-serine was produced from 100 mg/ml glycine and 88 mg/ml methanol in 3 d. The high l-serine degrading activity of the bacterium was entirely suppressed by adding an appropriate amount of CdCl2 (ca. 1 mM), resulting in an enhanced conversion ratio of glycine to l-serine (ca. 100% molar conversion). |
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