Selective modification of tryptophan-150 in ovine placental lactogen |
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| Institution: | 1. Department of Dermatology, The 2nd Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330006, China;2. Institute of Biomedical Innovation and School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330031, China;3. Department of Plastic, Medical Center of Burn Plastic and Wound Repair, The 1st Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330006, China;1. Department of Food Science, Aarhus University, Agro Food Park 48, DK-8200 Aarhus N, Denmark;2. Sino-Danish College (SDC), University of Chinese Academy of Science, Huairou District, Beijing 101408, China;3. Department of Health Science and Technology, Aalborg University, Selma Lagerløfsvej 249, DK-9260 Gistrup, Denmark;4. Clinical cancer center, Aalborg University Hospital, 9000 Aalborg, Denmark;1. Centre for Crop and Disease Management, Curtin University, Bentley, WA, Australia;2. Proteomics International, Nedlands, WA, Australia |
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| Abstract: | - 1.1. Ovine placental lactogen was modified by reaction with o-nitrophenylsulfenyl chloride. Fluorescence measurements indicated that one of the two tryptophan residues of the molecule had reacted. Besides, there was some reagent not covalently bound.
- 2.2. The reagent was covalently bound to Trp-150. No evidence of modification of Trp-90 was found.
- 3.3. Binding capacity to lactogenic as well as somatogenic receptors was diminished but not abolished upon modification, indicating that absolute molecular integrity of Trp-150 is not required for binding.
- 4.4. This behavior is similar to that of the tryptophan residues of ovine prolactin.
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