Production of cellulose from glucose by Acetobacter xylinum |
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| Institution: | 1. Central Laboratory, Rengo Co. Ltd., 4-1-186 Ohhiraki, Fukushima-ku, Osaka 553, Japan;2. The Osaka Municipal Technical Research Institute, 1-6-50 Morinomiya, Joto-ku, Osaka 536, Japan;1. LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon;2. PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon;3. Lyon University, Lyon1 University, IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Villeurbanne, France;4. Laboratory of Microbiology, Department of Biology, Lebanese University, Faculty of Sciences I, PO Box 5, Hadat, Lebanon;1. Ege University, Graduate School of Natural and Applied Science, Department of Biomedical Technologies, Izmir, Turkey;2. Ege University, Application and Research Center for Testing and Analysis (EGE-MATAL), Izmir, Turkey;3. Ege University, Department of Bioengineering, Faculty of Engineering, Izmir, Turkey |
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| Abstract: | Acetobacter xylinum 1FO 13693 was selected as the best cellulose-producing bacterium among 41 strains belonging to the genus Acetobacter and Agrobacterium. Cellulose was found to be produced at the liquid surface in static liquid cultivation. The rate of cellulose production depended proportionally on the surface-area of the culture medium and was unaffected by the depth and volume of the medium. The optimum pH for cellulose production was 4.0 to 6.0. Glucose, fructose and glycerol were preferred carbon sources for cellulose production. The yield of cellulose, relative to the glucose consumed, decreased with an increase in initial glucose concentration, and gluconic acid accumulated at a high initial glucose concentration. The decrease in cellulose yield could be due to some glucose being metabolized to gluconic acid. However, the accumulated gluconic acid did not affect cellulose production. The culture conditions of the bacterium for cellulose production were optimized. The maximum production rate of cellulose was 36 g/d·m2, with a yield of 100% for added glucose under the optimal conditions. |
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