Polymorphism of alpha-1 antitrypsin in dogs |
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Institution: | 1. Clinical Research Department, Mochida Pharmaceutical Co., Ltd., 1-22 Yotsuya, Shinjuku-ku, Tokyo, 160-0004, Japan (Mori and Yokoyama);2. Department of Cardiology, Tokyo Women''s Medical University, 8-1, Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan (Murasaki) |
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Abstract: | - 1.1. A genetically determined polymorphism of alpha-1 antitrypsin is demonstrated in dog serum by isoelectric focusing in a pH range of 3.5–5.0, followed by direct immunoblotting using a specific antiserum.
- 2.2. Alpha 1 antitrypsin focuses as two major bands at isoelectric points of 4.60 and 4.64 or 4.67 and 4.7 in presumed homozygous animals. Heterozygotes show both sets of bands.
- 3.3. The results of seven crosses with 33 offspring are best explained by two codominant alleles, PiM and PiS at a single locus designated as Pi for proteinase inhibitor.
- 4.4. The concentration of alpha-1 antitrypsin in serum of healthy dogs was 2.65 ± 0.42 mg/ml and 2.19 ± 0.38 mg/ml in females andv males respectively.
- 5.5. The higher concentration in female dogs suggests that estrogens may influence the serum level of alpha-1 antitrypsin.
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