Characteristics of plasmid stability in Bacillus subtilis NB22, an antifungal-antibiotic iturin producer |
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| Affiliation: | 1. Department of Chemical Engineering, Faculty of Engineering, University of Jember, Jember, Indonesia;2. Department of Agricultural Products Technology, Faculty of Agricultural Technology, University of Jember, Jember, Indonesia;3. Advanced Technology Development, Dexa Laboratories of Biomolecular Sciences (DLBS), PT Dexa Medica, Indonesia;1. Centre for Water Quality and Algae Research, Department of Zoology, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka;2. Faculty of Graduate Studies, University of Sri Jayewardenepura, Nugegoda 10250, Sri Lanka;3. Department of Environmental Technology, Faculty of Technology, University of Colombo, Sri Lanka |
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| Abstract: | The stability of plasmids pC194 and pUB110 was investigated in an antifungal antibiotic iturin producer, Bacillus subtilis NB22. Although plasmid pC194 was maintained stably over a hundred generations in five successive cultivations in iturin-production no. 3 medium, significant curing was observed when the cultivation was prolonged for 10 d in the same medium. When the transformant of pC194 was cultivated in Schaeffer's sporulation medium, drastic curing took place in accordance with the occurrence of sporulation, even in the presence of an antibiotic for selective pressure. In the case of pUB110, in sharp contrast to the result with pC194, high stability was observed in the sporulation medium, but significant curing was observed during prolonged incubation in no. 3 medium. |
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