Changes in serum immunoglobulin M (IgM) concentrations during early development of chum salmon (Oncorhynchus keta) as determined by sensitive elisa technique |
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| Institution: | 1. Department of Biological Sciences, University of Bergen, Norway;2. Woods Hole Oceanographic Institution, Woods Hole, MA, USA;3. Institute of Marine Research, Bergen, Norway;4. Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway;1. Animal Welfare Laboratory, Agricultural Sciences Sector, Federal University of Paraná, Brazil. Rua dos Funcionários 1540, Juvevê, Curitiba, Paraná CEP: 80035-050, Brazil;2. Research Station in Fish Farming and Ecology of Native Species of ITAIPU Binacional, Foz do Iguaçu, Paraná CEP: 85856-970, Brazil;1. University of Bergen, Department of Biology, Thormøhlensgate 53, Box N-7803, N-5020 Bergen, Norway;2. Institute of Marine Research, Box 1870 Nordnes, N-5817 Bergen, Norway;3. University of Bergen, Department of Physics and Technology, Allégaten 55, N-5007 Bergen, Norway;4. Nofima AS, Processing Technology Dept., Box 8034, N-4068 Stavanger, Norway |
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| Abstract: | - 1.1. A specific and sensitive sandwich enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of low levels of serum immunoglobulin M (IgM) of chum salmon Oncorhynchus keta.
- 2.2. In this assay, 5 μl serum was enough to measure the concentration of IgM and the minimum detectable concentration of serum IgM was about 5 ng/ml.
- 3.3. Coefficients of variation within and between assays ranged from 2.90 to 9.61%.
- 4.4. IgM concentrations remained at low level (< 300 ng/ml) until 40 days after hatching and then increased rapidly at the period of emergence (48 days after hatching).
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