An anticoagulant solution for haemolymph collection and prophenoloxidase studies of penaeid shrimp (Penaeus californiensis) |
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| Affiliation: | 1. Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, México;2. Centro de Investigación Facultad de Medicina UNAM-UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma ‘Benito Juárez’ de Oaxaca. Oaxaca de Juárez, Oaxaca, México;3. Facultad de Odontología, Universidad Autónoma ‘Benito Juárez’ de Oaxaca, Oaxaca de Juarez, Oaxaca, México;1. Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Belgium;2. Laboratory of Aquaculture & Artemia Reference Center, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Belgium |
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| Abstract: | - 1.1. An anticoagulant solution was designed from data on osmolality, ionic concentration and pH to resemble shrimp haemolymph.
- 2.2. This Shrimp Salt Solution (SSS) prevents coagulation and prophenoloxidase system activation during the extraction of shrimp haemolymph.
- 3.3. The location of the the proPO system in the brown shrimp (Penaeus californiensis) was determined using this anticoagulant solution.
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