Vicilin variants and the resistance of cowpea (Vigna unguiculata) seeds to the cowpea weevil (Callosobruchus maculatus) |
| |
| Affiliation: | 1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Liaoning Clinical Research Center for Lung Cancer, The Second Hospital of Dalian Medical University, Dalian 116023, China;1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China;2. Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Institute of Environment and Health, Jianghan University, Wuhan 430056, China;3. Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;1. Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697-4500, USA;2. Department of Animal and Environmental Biology, Federal University Oye-Ekiti, 371010 Ekiti State, Nigeria;3. Department of Plant Science and Biotechnology, Federal University Oye-Ekiti, 371010 Ekiti State, Nigeria;4. Laboratory website: http://sites.uci.edu/jameslab;1. HMP Leeds, Healthcare Department, 2 Gloucester Terrace, Armley, Leeds, LS12 2TJ, United Kingdom;2. Leeds Community Healthcare NHS Trust, Leeds, LS6 1PF, United Kingdom;3. Leeds Institute of Health Sciences, University of Leeds, LS2 9LJ, United Kingdom;1. Centre for Research in Agricultural Genomics (CRAG), Edifici CRAG, Campus UAB, 08193 Cerdanyola, Barcelona, Spain;2. Universitat de Girona, Institute for Food and Agricultural Technology (INTEA), Campus Montilivi (EPS-1), 17003 Girona, Spain;3. RIKILT Wageningen University & Research, Akkermaalsbos 2, Wageningen, The Netherlands;4. Council for Scientific and Industrial Research (CSIR), Biosciences, Brummeria, Pretoria, South Africa;5. Wageningen University & Research, Biometris, Wageningen, The Netherlands |
| |
| Abstract: | 1. A globulin fraction prepared from the meal of Callosobruchus maculatus-resistant cowpea (Vigna wiguiculata) seeds was shown to be detrimental to this bruchid when incorporated in artificial seeds.2. The performance of C. maculatus was also shown to be strongly hindered by vicilins from resistant seeds when these storage proteins were incorporated in artificial seeds at the level of 2%.3. The purified vicilins from seeds of both resistant and susceptible cowpea varieties were shown to have the same SDS-PAGE pattern but different mobilities in non-denaturing polyacrylamide gel electrophoresis.4. These results and previous ones obtained by us (Silva and Xavier-Filho, 1991; Sales et al., 1992) strongly suggest that the resistance of cowpea seeds from the cultivar TVu 2027 and from others bred from it is associated with the presence of vicilin molecules which are refractory to digestion by bruchid midgut proteinases. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
