The isolation of a plasma membrane-rich fraction from the skeletal muscle of two species of marine crab,Carcinus maenas and Cancer pagurus |
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Affiliation: | 1. Institut de Ciències del Mar (ICM–CSIC), Passeig Marítim de la Barceloneta 37–49, E-08003 Barcelona, Spain;2. CSIRO, Agricultural Productivity Flagship, Ecosciences Precinct, GPO Box 2583, Brisbane, 4001, Australia |
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Abstract: | - 1.1. A rapid method for the isolation of a plasma membrane-rich fraction from crab leg muscle, with high purity and yield recovery was developed.
- 2.2. The method is based on sodium iodide extraction of the crude homogenate, followed by centrifugation on Percoll self-creating gradient.
- 3.3. (Na+K+)ATPase and alkaline phosphodiesterase I were used as marker enzymes for the plasma membrane and revealed levels of purification of approximately 13-fold and yields recovery of the total activity in the crude muscle homogenate of approximately 18%, for both species of crab studied.
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