Arachidonic acid,protein kinase C activators and bud formation in Hydra vulgaris |
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| Institution: | 2. Istituto di Cibernetica, C.N.R. Via Toiano 6, 80072, Arco Felice, Naples, Italy;2. Istituto per la Chimica di Molecole di Interesse Biologico, C.N.R. Via Toiano 6, 80072, Arco Felice, Naples, Italy (Fax 081-804-1770);1. Centro de Investigación y Desarrollo en Tecnología de Pinturas-CIDEPINT, Calle 52 e/ 121 y 122, La Plata B1900AYB, Argentina;2. Universidad Nacional de La Plata, Facultad de Ciencias Naturales y Museo, Calle 60 y 122, 1900 La Plata, Argentina;3. Universidad de Buenos Aires, Departamento de Química Orgánica – Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina;4. CONICET-Universidad de Buenos Aires, Unidad de Microanálisis y Métodos Físicos en Química Orgánica (UMYMFOR), Buenos Aires, Argentina;5. Universidad Nacional de La Plata, Facultad de Ciencias Agrarias y Forestales, Calle 60 y 119, 1900 La Plata, Argentina;1. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA;2. Cellular Computations and Learning, Max Planck Institute for Neurobiology of Behavior – Caesar, Bonn, Germany;1. Computational Science Lab, University of Amsterdam, Science Park 904, 1098XH, Amsterdam, the Netherlands;2. Department of Evolutionary Biology, Lomonosov Moscow State University, Russia;3. Koltzov Institute of Developmental Biology of the Russian Academy of Sciences, 26 Vavilov Street, Moscow, 119334, Russia;4. Sorbonne Université, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), 06230, Villefranche-sur-mer, France |
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| Abstract: | 1. The effect of the protein kinase C (PKC) activators verrucosin B (VB), 1,2-sn-dioctanoylglycerol (diC8) and phorbol-12-myristate-13-acetate (PMA), of arachidonic acid (AA) and of substances interfering with its release, re-uptake and metabolism was studied in Hydra vulgaris.2. All PKC activators potently inhibited bud formation, VB and PMA being 10,000 × more potent than diC8. VB effect was maximal already after 10 min incubation with hydra and persisted at 24 hr incubations.3. AA and substances inhibiting its re-uptake from cell membrane or its metabolism also inhibited bud formation, whereas oleyl-oxyethyl-phosphorylcholine (OOPC), an inhibitor of phospholipase A2, potently induced bud formation.4. The findings described herein suggest a role for both PKC activation and AA in the inhibition of bud formation in H. vulgaris. |
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