Potassium channels in growth cones of leech retzius cells |
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| Institution: | 1. Department of Neuroscience, The University of Texas, Austin, Texas;2. Department of Integrative Biology, The University of Texas, Austin, Texas;3. Institute for Computational Molecular Science, College of Science and Technology & Institute for Genomics and Evolutionary Medicine, Temple University, Philadelphia, Pennsylvania;1. Department of Basic Medical Sciences, Medical School, Universidad de La Laguna, Tenerife, Spain;2. Instituto de Tecnologias Biomedicas, Universidad de La Laguna, Tenerife, Spain;1. School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom;2. Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, United Kingdom;3. Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom;4. Autifony Srl, Padova, Italy |
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| Abstract: | - 1.1. Potassium-selective channels were analysed in growth cones of cultured leech Retzius cells.
- 2.2. In the cell-attached mode and at physiological bath and pipette solution little channel activity was observed at resting membrane potential. The channel open probability (po) increased with cell depolarization, and the slope conductance of the single K+ channel current was about 60 pS.
- 3.3. With symmetrical high KCl solution on both sides of the excised membrane patch three K+ -selective channels could be discriminated. Two channels exhibited a linear current-voltage relation of about 18 pS and 106 pS, respectively.
- 4.4. The most frequently observed K+ channel showed a non-linear current-voltage relation and po increased with increasing free cytoplasmic Ca2+ and during cell hyperpolarization.
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