Detection of 180 kDa proteins in electroplax sodium channel preparations |
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| Institution: | 1. State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, Gansu Tech Innovation Center of Western China Grassland Industry, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;2. Department of Plant Biology, Rutgers University, New Brunswick, NJ 08901-8520, USA;3. Grassland Research Center of National Forestry and Grassland Administration, Chinese Academy of Forestry, Beijing 100091, China;1. Department of Medicine III & Center for Healthy Aging, Technische Universität Dresden, Germany;2. Department of Physiological Sciences, University of Florida Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, USA;3. Usher Institute, The University of Edinburgh, Edinburgh, UK;4. Department of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA |
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| Abstract: | - 1.1. Co-isolating proteins (Mr 170,000–220,000) from sodium channel preparations made from the electric organ of the electric eel (Electrophorus electricus) were detected on Western blots using monoclonal a antibodies.
- 2.2. Similar protein patterns were seen on immunoblots containing immunoprecipitated protein from eel muscle and brain tissues but not heart.
- 3.3. These co-isolating proteins could be separated from the mature TTX-sensitive channel protein (Mr 280,000) using a lentil lectin-Sepharose column.
- 4.4. The 180 kDa proteins do not appear to be channel-related and can be detected as contaminants in electroplax sodium channel preparations using the monoclonal antibodies described here.
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