Elasmobranch fish (Scyliorhinus canicula and Raja clavata) muscle AMP deaminase: Substrate specificity and effect of EDTA and adenylate energy charge

• 1.1. The AMP deaminases from skeletal muscles of dogfish and skate were shown to be specific to 5′-AMP. Among several adenine nucleotide analogs, only dAMP was deaminated to an extent lower than 5%.
• 2.2. Similar to vertebrates AMP deaminases, these enzymes were inhibited when incubated in the presence of EDTA solutions.
• 3.3. The activity of the enzymes was regulated by adenylic energy charge variations, depending on the size of the total adenine nucleotide pool.
• 4.4. The shape of the adenylate energy charge response curves of the dogfish and skate muscle AMP deaminases do not distinguish the two enzymes.