Monoclonal antibodies neutralizing the haemolytic activity of box jellyfish (Chironex fleckeri) tentacle extracts |
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| Affiliation: | 1. Institute for Baltic Sea Research, D 18119 Rostock-Warnemünde, Germany;2. Plankton Ecophysiology Group, Instituto de Oceanografía y Cambio Global, Universidad de Las Palmas de Gran Canaria, 35017 Canary Islands, Spain;1. Division of Cardiology, Department of Medical and Surgical Sciences, University Magna Graecia, Italy;2. URT CNR of IFC, University Magna Graecia, Italy |
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| Abstract: | - 1.1. Three monoclonal antibodies have been produced which neutralize in vitro the haemolytic activity present in tentacle extracts of the box jellyfish (Chironex fleckeri).
- 2.2. Two of these monoclonal antibodies bound specifically to a component of relative molecular mass 50,000 in tentacle extract on Western blots.
- 3.3. This binding only occurred when the extracts were electrophoresed under non-reducing conditions.
- 4.4. The third monoclonal antibody did not display binding to Western blots of tentacle extract under any of our experimental conditions.
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