In vitro substrate utilization for lipid synthesis in liver explants from hyperthyroid chickens |
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| Institution: | 2. Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Yangzhou, Jiangsu, 225125, China;3. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 225009, China;2. Poultry Research Foundation, The University of Sydney, Camden NSW 2570, Australia;3. Sydney School of Veterinary Science, The University of Sydney, Sydney NSW 2006, Australia;2. Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Executive Yuan, Pingtung 91201, Taiwan;3. School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan;4. Department of Animal Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan;6. Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan;5. The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan |
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| Abstract: | - 1.1. Indian River male broiler chickens growing from 7 to 28 days of age were fed diets containing 12, 18, 24 and 30% protein + 0 or 1 mg triiodothyronine (T3)/kg of diet to study energetic costs of lipogenesis and the use of various substrates for in vitro lipogenesis.
- 2.2. De novo lipid and CO2 production were determined in the presence of 1-14C]pyruvate, 2-14q]pyruvate, 3-14C]pyruvate, 2-14C]acetate and U-14C]alanine.
- 3.3. Oxygen consumption was determined in mitochondrial preparations to estimate the energetic costs in expiants synthesizing lipid.
- 4.4. Radiolabeled CO2 derived from 1-14C]pyruvate was used as an estimate of coenzyme A availability in liver expiants. Lipids derived from 2-14C]pyruvate, 2-14C]acetate and U-14C]alanine estimate relative substrate efficiency.
- 5.5. Labeled CO2 production from 1-14C]pyruvate was greatest in that group fed a 12% protein diet and least in the group fed a 30% protein diet.
- 6.6. In addition, T3 increased CO2 production from 1-14C]pyruvate.
- 7.7. The production of 14CO2 from the second carbon of pyruvate or acetate was increased by T3.
- 8.8. The low-protein diet (12% protein) increased (P <0.05) lipogenesis.
- 9.9. Adding T3 to the diets decreased carbon flux into lipid from all substrates, but increased CO2 production from all substrates without changing stage 3 and 4 respiration rates in mitochondrial preparations.
- 10.10. These observations imply that coenzyme A availability may have regulated de novo lipogenesis in the present study.
- 11.11. It was also concluded that previously noted effects of T3 on intermediary metabolism may involve metabolic pathways that do not involve changes in mitochondrial function.
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