Comparative study of proteolytic enzymes in the digestive tracts of the european sea bass and hybrid striped bass reared in freshwater |
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| Institution: | 1. Institute for Marine and Antarctic Studies, Fisheries and Aquaculture Centre, University of Tasmania, Hobart, Tasmania 7001, Australia;2. School of Biological Sciences and the Oceans Institute, The University of Western Australia, Crawley 6009, Australia;3. Department of Biological Sciences, Macquarie University, North Ryde, New South Wales 2109, Australia;4. School of Life Sciences, La Trobe University, Bundoora, Victoria 3086, Australia;5. Tropical Futures Institute, Aquaculture, James Cook University Singapore, S387380, Singapore |
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| Abstract: | - 1.1. A comparative study of the proteolytic activity in four different sections of the digestive tracts of the European sea bass (Dicentrarchus labrax) and hybrid striped bass (Morone chrysops × M. saxatilis) reared in freshwater revealed minor differences between these fish.
- 2.2. Tryptic activity plays a major role in the proteolytic process in both fish.
- 3.3. The activity of seven intestinal proteolytic enzymes was detected utilizing a combination of specific substrates and inhibitors.
- 4.4. High levels of proteolytic activity were detected in both the proximal and distal sections of the fish intestine at a high pH range (9–10).
- 5.5. In situ monitoring of pH levels revealed a lower pH level in the intestinal proximal section of hybrid striped bass compared with the distal section.
- 6.6. In contrast, higher pH levels were detected at the proximal compared with the distal sections of D. labrax intestine.
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