Fermentation characteristics of levansucrase mutants of Zymomonas mobilis |
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| Affiliation: | 1. Instrument Centre for Solid-State NMR Spectroscopy, Department of Chemistry and Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark;2. Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Concrete/Construction Chemistry, Überlandstrasse 129, 8600 Dübendorf, Switzerland;1. Nano and Bioelectrochemistry Research Laboratory, Carbon dioxide Research and Green Technology Centre, Vellore Institute of Technology University, Vellore, Tamil Nadu 632014, India;2. Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology University, Vellore 632014, India |
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| Abstract: | Two mutants, Ls1 and Ls2, of Zymomonas mobilis B-806 unable to produce levan were isolated. With native gel electrophoresis and zymogram analysis it was confirmed that the mutants did not synthesize active levansucrase (E2). However, they produced intracellular sucrase (E1) and extracellular invertase (E3). Comparison of these mutants with the parent strain for alcohol production on glucose, fructose and sucrose (100 g/l each) media revealed that the final ethanol concentration achieved in sucrose medium was only about 5 g/l higher with the mutants than with the wild type. The ethanol yield of the mutants increased from 0.48 g/g to 0.50 g/g on sucrose medium. |
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