Acidolysis of sardine oil by lipase to concentrate eicosapentaenoic and docosahexaenoic acids in glycerides |
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| Institution: | 1. Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 724, Japan;2. Ikeda Tohka Co. Ltd., 95-1 Minooki-cho, Fukuyama 721, Japan;1. College of Electrical and Electronic Engineering, Anhui Science and Technology University, Bengbu, 233030, People''s Republic of China;2. Key Laboratory of Materials Physics, Institute of Solid State Physics, University of Chinese Academy of Sciences, Hefei, 230031, People''s Republic of China;3. School of Physics and Materials Science, Anhui University, Hefei, 230601, People''s Republic of China;4. College of Chemistry and Materials Engineering, Anhui Science and Technology University, Bengbu, 233030, People''s Republic of China;2. Univ Lyon, Université Lyon 1, INSA Lyon, CPE Lyon, Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires, UMR 5246, Chimie Organique et Bioorganique, Bâtiment Jules Verne, 20 Avenue Albert Einstein, 69621 Villeurbanne Cedex, France;1. CNRS-Aix-Marseille Université-UMR7282 Enzymology at Interfaces and Physiology of Lipolysis, Marseille, France;2. Laboratoires Mayoly Spindler SAS, Chatou, France;3. Proteabio Europe SAS, Langlade, France;4. Wil Research Europe-Lyon, Saint-Germain Nuelle, France;5. AP-HM, Gastroenterology Department, La Timone Hospital, 13005 Marseille, France;1. School of Biotechnology and Key laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;2. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China |
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| Abstract: | Lipoprotein lipase from Pseudomonas sp. was the best enzyme to concentrate eicosapentaenoic and docosahexaenoic acids (EPA and DHA) in sardine oil by acidolysis reaction, and acetone was more effective than n-hexane as a solvent for dissolving the reactants and concentrating the two fatty acids. The water concentration in the reaction mixture was a decisive factor governing the enrichment of EPA and DHA and the yield of glycerides. EPA and DHA were more concentrated, but the yield of glycerides decreased, when the water concentration was increased gradually. Thus, the concentration rates of both the fatty acids were low with 0.25% water, although a considerable amount of diglyceride was detectable in the reaction products. The effect of reaction temperature was very slight with the use of acetone; however, the ratio DHA/EPA increased when the temperature was lowered in the presence of n-hexane. When acidolysis was performed at 25°C for 1 h, using 10,000 units of lipase per g of the reactants, the total percentage of EPA and DHA reached 65% in the glycerides and the recoveries of the two acids were 87.4 and 81.3%, respectively, based on the contents in the original sardine oil. The relationship of the enzyme substrate specificity to the reaction results was also investigated. |
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