31P-NMR study on effects of electric shock on swimming leeches,Hirudo medicinalis |
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| Institution: | 1. Department of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran;2. Department of Mechanical Engineering, Babol University of Technology, Babol 484, Iran |
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| Abstract: | - 1.1. We evaluated the effect of electric shock on swimming leeches by measuring changes in high-energy phosphate metabolism using in vivo31P-NMR.
- 2.2. Leeches electrically stimulated during swimming showed anodal galvanotaxis, and stopped swimming with stimulation at strong current.
- 3.3. Comparison of the concentrations of high-energy phosphate metabolites before and after electric shock using 31P-NMR revealed a marked decrease in β-ATP and an increase in that of Pi.
- 4.4. Electric shock apparently induces excessive muscle fatigue in leeches, resulting in transient paralysis.
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