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A combination immunoblot for the simultaneous detection and differentiation of HIV-1 and HIV-2
Institution:1. Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, China;2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China;3. University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Pakistan;1. Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA;2. Memorial Sloan Kettering Cancer Center, New York, NY;1. Research Center for Evidence-Based Medicine, Health Management and Safety Promotion Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran;2. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran;3. Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;4. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;1. Department of Hematology and Clinical Immunology, Yokohama City University School of Medicine, Yokohama, Japan;2. Department of Hematology, Yokohama City University Medical Center, Yokohama, Japan;3. Department of Hematology, Kanagawa Cancer Center, Yokohama, Japan;4. Department of Hematology, Shizuoka Red Cross Hospital, Shizuoka, Japan;5. Department of Hematology/Oncology, Yamato Municipal Hospital, Yamato, Japan;6. Department of Hematology, Fujisawa City Hospital, Fujisawa, Japan;7. Department of Hematology, Saiseikai Yokohama Nanbu Hospital, Yokohama, Japan
Abstract:We have developed a combination immunoblot (combi-blot) for simultaneous detection and differentiation of HIV-1 and HIV-2 antibodies using HIV-1 lysate and HIV-1 and HIV-2 synthetic peptide antigens in a single assay. Minimal modification in antigen strip preparation and no modification in assay procedure of the current HIV-1 Western blot protocol was required. Implementation of this combi-blot following the use of the combination HIV-1/-2 enzyme immunoassay would simplify the current HIV testing algorithm and increase the accuracy of HIV-2 surveillance.
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